0587538 - ÚEM 2025 RIV US eng J - Článek v odborném periodiku
Fabbri, R. - Scidá, A. - Saracino, E. - Conte, G. - Kovtun, A. - Candini, A. - Kirdajová, Denisa - Spennato, D. - Marchetti, Valeria - Lazzarini, Ch. - Konstantoulaki, A. - Dambruoso, P. - Caprini, M. - Muccini, M. - Ursino, M. - Anděrová, Miroslava - Treossi, E. - Zamboni, R. - Palermo, V. - Benfenati, V.
Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes.
Nature Nanotechnology. ISSN 1748-3387. E-ISSN 1748-3395
Grant CEP: GA ČR(CZ) GA20-05770S; GA MŠMT(CZ) LM2023050
GRANT EU: European Commission(XE) 956325 - TUBE
Institucionální podpora: RVO:68378041
Obor OECD: Neurosciences (including psychophysiology

Astrocytes are responsible for maintaining homoeostasis and cognitive
functions through calcium signalling, a process that is altered in brain
diseases. Current bioelectronic tools are designed to study neurons and are
not suitable for controlling calcium signals in astrocytes. Here, we show that
electrical stimulation of astrocytes using electrodes coated with graphene
oxide and reduced graphene oxide induces respectively a slow response to
calcium, mediated by external calcium influx, and a sharp one, exclusively
due to calcium release from intracellular stores. Our results suggest that the
different conductivities of the substrate influence the electric field at the
cell–electrolyte or cell–material interfaces, favouring different signalling
events in vitro and ex vivo. Patch-clamp, voltage-sensitive dye and calcium
imaging data support the proposed model. In summary, we provide
evidence of a simple tool to selectively control distinct calcium signals in
brain astrocytes for straightforward investigations in neuroscience and
bioelectronic medicine.
Trvalý link: https://hdl.handle.net/11104/0354672