0587089 - ÚOCHB 2025 RIV DE eng J - Článek v odborném periodiku
Krömer, Matouš - Poštová Slavětínská, Lenka - Hocek, Michal
Glyco-DNA: Enzymatic Synthesis of Base-modified and Hypermodified DNA Displaying up to Four Different Monosaccharide Units in the Major Groove.
Chemistry - A European Journal. Roč. 30, č. 48 (2024), č. článku e202402318. ISSN 0947-6539. E-ISSN 1521-3765
Grant CEP: GA ČR(CZ) GX20-00885X
Institucionální podpora: RVO:61388963
Klíčová slova: DNA synthesis * nucleotides * carbohydrates * lectins * glycopolymers
Obor OECD: Organic chemistry
Impakt faktor: 3.9, rok: 2023 ; AIS: 0.89, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://doi.org/10.1002/chem.202402318DOI: https://doi.org/10.1002/chem.202402318

A portfolio of six modified 2'-deoxyribonucleoside triphosphate (dNTP) derivatives derived from 5-substituted pyrimidine or 7- substituted 7-deazapurine bearing different carbohydrate units (D-glucose, D-galactose, D-mannose, L-fucose, sialic acid and N-Ac-Dgalactosamine) tethered through propargyl-glycoside linker was designed and synthesized via the Sonogashira reactions of halogenated dNTPs with the corresponding propargyl-glycosides. The nucleotides were found to be good substrates for DNA polymerases in enzymatic primer extension and PCR synthesis of modified and hypermodified DNA displaying up to four different sugars. Proof of concept binding study of sugar-modified oligonucleotides with concanavalin A showed positive effect of avidity and sugar units count.
Trvalý link: https://hdl.handle.net/11104/0354386