Identification of Aspergillus Species using CE in Capillary with Roughened Part and MALDI TOF MS

0587079 - ÚIACH 2025 CZ eng A - Abstrakt
Šalplachta, Jiří - Kubesová, Anna - Karásek, Pavel - Růžička, M. - Roth, Michal
Identification of Aspergillus Species using CE in Capillary with Roughened Part and MALDI TOF MS.
MSB 2024. Final Program and Abstract Book. Brno: Ústav analytické chemie AV ČR, v. v. i., 2024 - (Lavická, J.; Přikryl, J.; Foret, F.). s. 172-172. ISBN 978-80-908154-1-4.
[MSB 2024. International Symposium on Microscale Separation and Bioanalysis /40./. 19.05.2024-22.05.2024, Brno]
Grant CEP: GA MZd(CZ) NU22-05-00110
Institucionální podpora: RVO:68081715
Klíčová slova: MALDI-TOF MS * Aspergillus species * used silica capillary
Obor OECD: Analytical chemistry
https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf

The aim of this study was to develop a method for identification of Aspergillus spp. in clinical samples based on a combination of capillary electrophoresis with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Aspergillus spp. conidia were on-line concentrated after their dynamic adhesion onto the roughened part of fused silica capillary (prepared by etching of the inner capillary surface with supercritical water) and subsequently off-line identified by MALDI-TOF MS. Conidia of four Aspergillus spp., A. niger, A. fumigatus, A. flavus, and A. parasiticus, were selected for this work.
Trvalý link: https://hdl.handle.net/11104/0354380