Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69

0587057 - ÚEB 2025 RIV US eng J - Článek v odborném periodiku
Li, Y. - Wei, Z. - Sela, H. - Govta, L. - Klymiuk, V. - Roychowdhury, R. - Chawla, H. S. - Ens, J. - Wiebe, K. - Bocharova, V. - Ben-David, R. - Pawar, P. B. - Zhang, Y. - Jaiwar, S. - Molnár, István - Doležel, Jaroslav - Coaker, G. - Pozniak, C. - Fahima, T.
Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69.
PLANT COMMUNICATIONS. Roč. 5, č. 1 (2024), č. článku 100646. ISSN 2590-3462
Grant CEP: GA MŠMT(CZ) EF16_019/0000827
Institucionální podpora: RVO:61389030
Klíčová slova: POWDERY MILDEW RESISTANCE * WILD EMMER WHEAT * DISEASE-RESISTANCE
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 10.5, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1016/j.xplc.2023.100646

Gene cloning in repeat-rich polyploid genomes remains challenging. Here, we describe a strategy for overcoming major bottlenecks in cloning of the powdery mildew resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat. A conventional positional cloning approach was not effective owing to suppressed recombination. Chromosome sorting was compromised by insufficient purity. A Pm69 physical map, constructed by assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed a rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. A single candidate NLR was identified by anchoring RNA sequencing reads from susceptible mutants to ONT contigs and was validated by virus-induced gene silencing. Pm69 is likely a newly evolved NLR and was discovered in only one location across the wild emmer wheat distribution range in Israel. Pm69 was successfully introgressed into cultivated wheat, and a diagnostic molecular marker was used to accelerate its deployment and pyramiding with other R-genes.
Trvalý link: https://hdl.handle.net/11104/0354360