0585892 - ÚMCH 2025 RIV US eng J - Článek v odborném periodiku
Sansee, A. - Kostka, Libor - Marcalíková, Adéla - Kudláčová, Júlia - Sedlák, František - Kotrchová, Lenka - Šácha, Pavel - Etrych, Tomáš - Kielar, F.
Iridium-based polymeric multifunctional imaging tools for biochemistry.
ChemPlusChem. Roč. 89, č. 5 (2024), č. článku e202300647. ISSN 2192-6506. E-ISSN 2192-6506
Grant CEP: GA ČR(CZ) GA23-05642S; GA MŠMT LX22NPO5102
Institucionální podpora: RVO:61389013 ; RVO:61388963
Klíčová slova: HPMA * iridium complex * imaging
Obor OECD: Polymer science; Medicinal chemistry (UOCHB-X)
Impakt faktor: 3, rok: 2023
Způsob publikování: Open access
Web výsledku:https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cplu.202300647DOI: https://doi.org/10.1002/cplu.202300647

Herein, we report the development of a macromolecular multifunctional imaging tool for biological investigations, which is comprised of an N-(2-hydroxypropyl)methacrylamide backbone, iridium-based luminescent probe, glutamate carboxypeptidase II (GCPII) targeting ligand, and biotin affinity tag. The iridium luminophore is a tris-cyclometalated complex based on [Ir(ppy)3] with one of its 2-phenylpyridine ligands functionalized to allow conjugation. Synthesized macromolecular probes differed in the structure of the polymer and content of the iridium complex. The applicability of the developed imaging tools has been tested in flow cytometry (FACS) based assay, laser confocal microscopy, and fluorescence lifetime imaging microscopy (FLIM). The FACS analysis has shown that the targeted iBodies containing the iridium luminophore exhibit selective labelling of GCPII expressing cells. This observation was also confirmed in the imaging experiments with laser confocal microscopy. The FLIM experiment has shown that the iBodies with the iridium label exhibit a lifetime greater than 100 ns, which distinguishes them from typically used systems labelled with organic fluorophores exhibiting short fluorescence lifetimes. The results of this investigation indicate that the system exhibits interesting properties, which supports the development of additional biological tools utilizing the key components (iridium complexes, iBody concept), primarily focusing on the longer lifetime of the iridium emitter.
Trvalý link: https://hdl.handle.net/11104/0353702