Počet záznamů: 1  

In situ separation and visualization of isomeric auxin derivatives in Arabidopsis by ion mobility mass spectrometry imaging

  1. 1.
    0585644 - ÚEB 2025 RIV DE eng J - Článek v odborném periodiku
    Zhang, Chao - Bieleszová, K. - Žukauskaitė, A. - Hladík, Pavel - Grúz, J. - Novák, Ondřej - Doležal, Karel
    In situ separation and visualization of isomeric auxin derivatives in Arabidopsis by ion mobility mass spectrometry imaging.
    Analytical and Bioanalytical Chemistry. Roč. 416, č. 1 (2024), s. 125-139. ISSN 1618-2642. E-ISSN 1618-2650
    Institucionální podpora: RVO:61389030
    Klíčová slova: Auxin * Desorption electrospray ionization * Ion mobility * Isomer * Mass spectrometry imaging * Metabolite
    Obor OECD: Analytical chemistry
    Impakt faktor: 4.3, rok: 2022
    Způsob publikování: Open access
    https://doi.org/10.1007/s00216-023-04996-x

    In situ separation and visualization of synthetic and naturally occurring isomers from heterogeneous plant tissues, especially when they share similar molecular structures, are a challenging task. In this study, we combined the ion mobility separation with desorption electrospray ionization mass spectrometry imaging (DESI-IM-MSI) to achieve a direct separation and visualization of two synthetic auxin derivatives, auxinole and its structural isomer 4pTb-MeIAA, as well as endogenous auxins from Arabidopsis samples. Distinct distribution of these synthetic isomers and endogenous auxins in Arabidopsis primary roots and hypocotyls was achieved in the same imaging analysis from both individually treated and cotreated samples. We also observed putative metabolites of synthetic auxin derivatives, i.e. auxinole amino acid conjugates and hydrolysed 4pTb-MeIAA product — 4pTb-IAA, based on their unique drifting ion intensity patterns. Furthermore, DESI-IM-MSI-revealed abundance of endogenous auxins and synthetic isomers was validated by liquid chromatography–mass spectrometry (LC-MS). Our results demonstrate that DESI-IM-MSI could be used as a robust technique for detecting endogenous and exogenous isomers and provide a spatiotemporal evaluation of hormonomics profiles in plants.
    Trvalý link: https://hdl.handle.net/11104/0353326

     
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Počet záznamů: 1  

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