Heteromorphic ZZ/ZW sex chromosomes sharing gene content with mammalian XX/XY are conserved in Madagascan chameleons of the genus iFurcifer/i

0585092 - ÚŽFG 2025 RIV US eng J - Článek v odborném periodiku
Rovatsos, M. - Mazzoleni, S. - Augstenová, B. - Altmanová, Marie - Velenský, P. - Glaw, F. - Sánchez, A. - Kratochvíl, L.
Heteromorphic ZZ/ZW sex chromosomes sharing gene content with mammalian XX/XY are conserved in Madagascan chameleons of the genus iFurcifer/i.
Scientific Reports. Roč. 14, č. 1 (2024), č. článku 4898. ISSN 2045-2322. E-ISSN 2045-2322
Institucionální podpora: RVO:67985904
Klíčová slova: Homology * Karyotypes * Microdissection * Sex chromosomes * qPCR * Chameleons
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impakt faktor: 4.6, rok: 2022
Způsob publikování: Open access
https://www.nature.com/articles/s41598-024-55431-9

Chameleons are well-known lizards with unique morphology and physiology, but their sex determination has remained poorly studied. Madagascan chameleons of the genus Furcifer have cytogenetically distinct Z and W sex chromosomes and occasionally Z(1)Z(1)Z(2)Z(2)/Z(1)Z(2)W multiple neo-sex chromosomes. To identify the gene content of their sex chromosomes, we microdissected and sequenced the sex chromosomes of F. oustaleti (ZZ/ZW) and F. pardalis (Z(1)Z(1)Z(2)Z(2)/Z(1)Z(2)W). In addition, we sequenced the genomes of a male and a female of F. lateralis (ZZ/ZW) and F. pardalis and performed a comparative coverage analysis between the sexes. Despite the notable heteromorphy and distinctiveness in heterochromatin content, the Z and W sex chromosomes share approximately 90% of their gene content. This finding demonstrates poor correlation of the degree of differentiation of sex chromosomes at the cytogenetic and gene level. The test of homology based on the comparison of gene copy number variation revealed that female heterogamety with differentiated sex chromosomes remained stable in the genus Furcifer for at least 20 million years. These chameleons co-opted for the role of sex chromosomes the same genomic region as viviparous mammals, lacertids and geckos of the genus Paroedura, which makes these groups excellent model for studies of convergent and divergent evolution of sex chromosomes.
Trvalý link: https://hdl.handle.net/11104/0352908