Peptidoglycan Endopeptidase from Novel Adaiavirus Bacteriophage Lyses Pseudomonas aeruginosa Strains as Well as Arthrobacter globiformis and A. pascens Bacteria

0584343 - BC 2024 RIV CH eng J - Článek v odborném periodiku
Petrzik, Karel
Peptidoglycan Endopeptidase from Novel Adaiavirus Bacteriophage Lyses Pseudomonas aeruginosa Strains as Well as Arthrobacter globiformis and A. pascens Bacteria.
Microorganisms. Roč. 11, č. 8 (2023), č. článku 1888. E-ISSN 2076-2607
Grant CEP: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775
Grant ostatní: AV ČR(CZ) StrategieAV21/21
Program: StrategieAV
Institucionální podpora: RVO:60077344
Klíčová slova: adaiavirus * endolysin * host range * G plus and G- activity
Obor OECD: Virology
Impakt faktor: 4.5, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2076-2607/11/8/1888

A novel virus lytic for Pseudomonas aeruginosa has been purified. Its viral particles have a siphoviral morphology with a head 60 nm in diameter and a noncontractile tail 184 nm long. The dsDNA genome consists of 16,449 bp, has cohesive 3' termini, and encodes 28 putative proteins in a single strain. The peptidoglycan endopeptidase encoded by ORF 16 was found to be the lytic enzyme of this virus. The recombinant, purified enzyme was active up to 55 C-? in the pH range 6-9 against all tested isolates of P. aeruginosa, but, surprisingly, also against the distant Gram-positive micrococci Arthrobacter globiformis and A. pascens. Both this virus and its endolysin are further candidates for possible treatment against P. aeruginosa and probably also other bacteria.
Trvalý link: https://hdl.handle.net/11104/0352333