Holocentromeres can consist of merely a few megabase-sized satellite arrays

0584244 - BC 2024 RIV US eng J - Článek v odborném periodiku
Kuo, Y. T. - Câmara, A. S. - Schubert, V. - Neumann, Pavel - Macas, Jiří - Melzer, M. - Chen, J. - Fuchs, J. - Abel, S. - Klocke, E. - Huettel, B. - Himmelbach, A. - Demidov, D. - Dunemann, F. - Mascher, M. - Ishii, T. - Marques, A. - Houben, A.
Holocentromeres can consist of merely a few megabase-sized satellite arrays.
Nature Communications. Roč. 14, č. 1 (2023), č. článku 3502. E-ISSN 2041-1723
Grant CEP: GA ČR(CZ) GA20-25440S
Institucionální podpora: RVO:60077344
Klíčová slova: nuclear-dna content * histone h3 * chionographis liliaceae * luzula-elegans * centromere * chromosomes * arabidopsis * methylation * euchromatin * generation
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 16.6, rok: 2022
Způsob publikování: Open access
https://www.nature.com/articles/s41467-023-38922-7

The centromere is the chromosome region where microtubules attach during cell division. In contrast to monocentric chromosomes with one centromere, holocentric species usually distribute hundreds of centromere units along the entire chromatid. We assembled the chromosome-scale reference genome and analyzed the holocentromere and (epi)genome organization of the lilioid Chionographis japonica. Remarkably, each of its holocentric chromatids consists of only 7 to 11 evenly spacedmegabase-sized centromere-specific histone H3-positive units. These units contain satellite arrays of 23 and 28 bp-long monomers capable of forming palindromic structures. Like monocentric species, C. japonica forms clustered centromeres in chromocenters at interphase. In addition, the large-scale eu- and heterochromatin arrangement differs between C. japonica and other known holocentric species. Finally, using polymer simulations, we model the formation of prometaphase line-like holocentromeres from interphase centromere clusters. Our findings broaden the knowledge about centromere diversity, showing that holocentricity is not restricted to species with numerous and small centromere units.
Trvalý link: https://hdl.handle.net/11104/0352321