Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells

0583915 - FGÚ 2025 RIV CH eng J - Článek v odborném periodiku
Trávníčková, Martina - Filová, Elena - Slepička, P. - Kasálková-Slepičková, N. - Kocourek, Tomáš - Žaloudková, Margit - Suchý, Tomáš - Bačáková, Lucie
Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells.
International Journal of Molecular Sciences. Roč. 25, č. 5 (2024), č. článku 2837. ISSN 1661-6596
Grant CEP: GA ČR(CZ) GA20-01570S; GA MŠMT(CZ) EH22_008/0004562
Grant ostatní: AV ČR(CZ) AP2202
Program: Akademická prémie - Praemium Academiae
Institucionální podpora: RVO:67985823 ; RVO:68378271 ; RVO:67985891
Klíčová slova: titanium * diamond-like carbon layer (DLC) * biocompatibility * osteogenic differentiation * adipose tissue-derived stem cells (ADSCs) * bone marrow mesenchymal stem cells (bmMSCs)
Obor OECD: Orthopaedics; Coating and films (FZU-D); Biomaterials (as related to medical implants, devices, sensors) (USMH-B)
Impakt faktor: 5.6, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/25/5/2837

Diamond-like carbon (DLC) layers are known for their high corrosion and wear resistance, low friction, and high biocompatibility. However, it is often necessary to dope DLC layers with additional chemical elements to strengthen their adhesion to the substrate. Ti-DLC layers (doped with 0.4, 2.1, 3.7, 6.6, and 12.8 at.% of Ti) were prepared by dual pulsed laser deposition, and pure DLC, glass, and polystyrene (PS) were used as controls. In vitro cell–material interactions were investigated with an emphasis on cell adhesion, proliferation, and osteogenic differentiation. We observed slightly increasing roughness and contact angle and decreasing surface free energy on Ti-DLC layers with increasing Ti content. Three-week biological experiments were performed using adipose tissue-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (bmMSCs) in vitro. The cell proliferation activity was similar or slightly higher on the Ti-doped materials than on glass and PS. Osteogenic cell differentiation on all materials was proved by collagen and osteocalcin production, ALP activity, and Ca deposition. The bmMSCs exhibited greater initial proliferation potential and an earlier onset of osteogenic differentiation than the ADSCs. The ADSCs showed a slightly higher formation of focal adhesions, higher metabolic activity, and Ca deposition with increasing Ti content.
Trvalý link: https://hdl.handle.net/11104/0351899


Vědecká data: Zenodo