More than just an eagle killer: The freshwater cyanobacterium iAetokthonos hydrillicola/i produces highly toxic dolastatin derivatives

0583653 - BC 2024 RIV US eng J - Článek v odborném periodiku
Schwark, M. - Yerena, Jose Alberto Martinez - Roehrborn, K. - Hrouzek, P. - Divoká, P. - Štenclová, Lenka - Delawská, K. - Enke, H. - Vorreiter, C. - Wiley, F. - Sippl, W. - Sobotka, R. - Saha, S. - Wilde, S. B. - Mareš, Jan - Niedermeyer, T. H. J.
More than just an eagle killer: The freshwater cyanobacterium iAetokthonos hydrillicola/i produces highly toxic dolastatin derivatives.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 120, č. 40 (2023), č. článku e2219230120. ISSN 0027-8424. E-ISSN 1091-6490
Grant CEP: GA ČR(CZ) GC19-21649J
Institucionální podpora: RVO:60077344
Klíčová slova: antineoplastic agents * anticancer * inhibition * tubulin * cyanotoxin * cytotoxicity * aetokthonostatin * dolastatin
Obor OECD: Marine biology, freshwater biology, limnology
Impakt faktor: 11.1, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1073/pnas.2219230120

Cyanobacteria are infamous producers of toxins. While the toxic potential of planktonic cyanobacterial blooms is well documented, the ecosystem level effects of toxigenic benthic and epiphytic cyanobacteria are an understudied threat. The freshwater epiphytic cyanobacterium Aetokthonos hydrillicola has recently been shown to produce the 'eagle killer' neurotoxin aetokthonotoxin (AETX) causing the fatal neurological disease vacuolar myelinopathy. The disease affects a wide array of wildlife in the southeastern United States, most notably waterfowl and birds of prey, including the bald eagle. In an assay for cytotoxicity, we found the crude extract of the cyanobacterium to be much more potent than pure AETX, prompting further investigation. Here, we describe the isolation and structure elucidation of the aetokthonostatins (AESTs), linear peptides belonging to the dolastatin compound family, featuring a unique modification of the C-terminal phenylalanine-derived moiety. Using immunofluorescence microscopy and molecular modeling, we confirmed that AEST potently impacts microtubule dynamics and can bind to tubulin in a similar matter as dolastatin 10. We also show that AEST inhibits reproduction of the nematode Caenorhabditis elegans. Bioinformatic analysis revealed the AEST biosynthetic gene cluster encoding a non-ribosomal peptide synthetase/polyketide synthase accompanied by a unique tailoring machinery. The biosynthetic activity of a specific N-terminal methyltransferase was confirmed by in vitro biochemical studies, establishing a mechanistic link between the gene cluster and its product.
Trvalý link: https://hdl.handle.net/11104/0351681