Genomic survey maps differences in the molecular complement of vesicle formation machinery between iGiardia intestinalis/i assemblages

0583491 - BC 2024 RIV US eng J - Článek v odborném periodiku
Pipaliya, S. - Dacks, Joel Bryan - Croxen, M.A.
Genomic survey maps differences in the molecular complement of vesicle formation machinery between iGiardia intestinalis/i assemblages.
PLoS Neglected Tropical Diseases. Roč. 17, č. 12 (2023), č. článku e0011837. ISSN 1935-2735. E-ISSN 1935-2735
Institucionální podpora: RVO:60077344
Klíčová slova: endoplasmic-reticulum * escrt machinery * lamblia * duodenalis * identification * epidemiology * neogenesis * organelle * subunit * cell
Obor OECD: Infectious Diseases
Impakt faktor: 3.8, rok: 2022
Způsob publikování: Open access
https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011837

Giardia intestinalis is a globally important microbial pathogen with considerable public health, agricultural, and economic burden. Genome sequencing and comparative analyses have elucidated G. intestinalis to be a taxonomically diverse species consisting of at least eight different sub-types (assemblages A-H) that can infect a great variety of animal hosts, including humans. The best studied of these are assemblages A and B which have a broad host range and have zoonotic transmissibility towards humans where clinical Giardiasis can range from asymptomatic to diarrheal disease. Epidemiological surveys as well as previous molecular investigations have pointed towards critical genomic level differences within numerous molecular pathways and families of parasite virulence factors within assemblage A and B isolates. In this study, we explored the necessary machinery for the formation of vesicles and cargo transport in 89 Canadian isolates of assemblage A and B G. intestinalis. Considerable variability within the molecular complement of the endolysosomal ESCRT protein machinery, adaptor coat protein complexes, and ARF regulatory system have previously been reported. Here, we confirm inter-assemblage, but find no intra-assemblage variation within the trafficking systems examined. This variation includes losses of subunits belonging to the ESCRTIII as well as novel lineage specific duplications in components of the COPII machinery, ARF1, and ARFGEF families (BIG and CYTH). Since differences in disease manifestation between assemblages A and B have been controversially reported, our findings may well have clinical implications and even taxonomic, as the membrane trafficking system underpin parasite survival, pathogenesis, and propagation.
Trvalý link: https://hdl.handle.net/11104/0351452