Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action

0583389 - BTÚ 2024 RIV US eng J - Článek v odborném periodiku
Untergasser, A. - Hellemans, J. - Pfaffl, M.W. - Ruijter, J. M. - van den Hoff, M. J. B. - Dragomir, M. P. - Adamoski, D. - Dias, S. M. G. - Reis, R. M. - Ferracin, M. - Dias-Neto, E. - Marsh, I. - Kubista, Mikael - Fabbri, M. - Goel, A. - Slabý, O. - Knutsen, E. - Chen, B. - Negrini, M. - Mimori, K. - Pichler, M. - Papatriantafyllou, M. - Anfossi, S. - Schmittgen, T. D. - Huggett, J. - Bustin, S. - Vandesompele, J. - Calin, G. A. … celkem 29 autorů
Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action.
Molecular Oncology. Roč. 17, č. 5 (2023), s. 713-717. ISSN 1574-7891. E-ISSN 1878-0261
Institucionální podpora: RVO:86652036
Klíčová slova: guidelines * accuracy * quantification * rna * RT-qPCR
Obor OECD: Oncology
Impakt faktor: 6.6, rok: 2022
Způsob publikování: Open access
https://febs.onlinelibrary.wiley.com/doi/epdf/10.1002/1878-0261.13418

Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text), (b) submission of RT-qPCR raw data for all experiments reported, and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
Trvalý link: https://hdl.handle.net/11104/0351360