Influence of standard culture conditions and effect of oleoresin from the microalga iHaematococcus pluvialis/i on splenic cells from healthy Balb/c mice a pilot study

0583141 - MBÚ 2024 RIV US eng J - Článek v odborném periodiku
Jurčacková, Z. - Ciglanová, D. - Mudroňová, D. - Bárcenas-Pérez, Daniela - Cheel, José - Hrčková, G.
Influence of standard culture conditions and effect of oleoresin from the microalga iHaematococcus pluvialis/i on splenic cells from healthy Balb/c mice a pilot study.
In Vitro - Cellular and Developmental Biology. Animal Section. Roč. 59, č. 10 (2023), s. 764-777. ISSN 1071-2690. E-ISSN 1543-706X
Institucionální podpora: RVO:61388971
Klíčová slova: Standard culture conditions * Heamatococcus pluvialis * Oleoresin * Oxidative stress * Mouse spleen cells
Obor OECD: Cell biology
Impakt faktor: 2.1, rok: 2022
Způsob publikování: Open access
https://link.springer.com/article/10.1007/s11626-023-00822-x

In this work, we used splenocytes from healthy mice to study the effects of the two most commonly used cell culture media (A, B) with different compositions of redox reagents. The incubation of cells for 24 h resulted in a significant decrease in viability and metabolic activity of splenocytes, and the negative effects of incubation in medium B were more pronounced. In standard conditions, oxidative stress in cells was manifested by reduced mitochondrial potential, and this effect correlated with the transition of 58.3% of cells to the early stage of apoptosis under reducing conditions of medium A and up to 66.1% of cells under super-reducing conditions in medium B, suggesting altered cell physiology. High levels of ROS/RNS activated transcription factor Nrf2, superoxide dismutase 1, and catalase. The higher mRNA levels of these genes were under the conditions of medium B, whose super-reducing environment in combination with the environment of conventional incubators proved to be less suitable for the cells compared to medium A. Treatment of the cells with a lower concentration (10 mu g/ml) of oleoresin obtained from the microalga H. pluvialis partially eliminated the negative effects of cultivation. Higher concentration of oleoresin (40 mu g/ml) was slightly cytotoxic, due to the significant antioxidant effect of astaxanthin, the main bioactive component of the extract, which eliminated most of the ROS/RNS acting as signalling molecules. This study shows that the standard culture conditions do not reflect the physiological in vivo cell conditions, therefore, they are not generally suitable for incubation of all cell types.
Trvalý link: https://hdl.handle.net/11104/0351131