Microtubules under mechanical pressure can breach dense actin networks

0583048 - BTÚ 2024 RIV GB eng J - Článek v odborném periodiku
Gelin, M. - Schaeffer, A. - Gaillard, J. - Guerin, C. - Vianay, B. - Orhant-Prioux, M. - Braun, Marcus - Leterrier, C. - Blanchoin, L. - Thery, M.
Microtubules under mechanical pressure can breach dense actin networks.
Journal of Cell Science. Roč. 136, č. 22 (2023), č. článku jcs261667. ISSN 0021-9533. E-ISSN 1477-9137
Institucionální podpora: RVO:86652036
Klíčová slova: dynamic instability * growth * migration * complex * protein * populations * nucleation * filopodia * filaments * Lipid
Obor OECD: Cell biology
Impakt faktor: 4, rok: 2022
Způsob publikování: Open access
https://journals.biologists.com/jcs/article/136/22/jcs261667/335502/Microtubules-under-mechanical-pressure-can-breach

The crosstalk between the actin network and microtubules is essential for cell polarity. It orchestrates microtubule organization within the cell, driven by the asymmetry of actin architecture along the cell periphery. The physical intertwining of these networks regulates spatial organization and force distribution in the microtubule network. Although their biochemical interactions are becoming clearer, the mechanical aspects remain less understood. To explore this mechanical interplay, we developed an in vitro reconstitution assay to investigate how dynamic microtubules interact with various actin filament structures. Our findings revealed that microtubules can align and move along linear actin filament bundles through polymerization force. However, they are unable to pass through when encountering dense branched actin meshworks, similar to those present in the lamellipodium along the periphery of the cell. Interestingly, immobilizing microtubules through crosslinking with actin or other means allow the buildup of pressure, enabling them to breach these dense actin barriers. This mechanism offers insights into microtubule progression towards the cell periphery, with them overcoming obstacles within the denser parts of the actin network and ultimately contributing to cell polarity establishment.
Trvalý link: https://hdl.handle.net/11104/0351067