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A comparative assessment of a piezoelectric biosensor based on a new antifouling nanolayer and cultivation methods: Enhancing S. aureus detection in fresh dairy products
- 1.0582173 - FZÚ 2024 RIV NL eng J - Článek v odborném periodiku
Forinová, Michala - Seidlová, A. - Pilipenco, Alina - Lynn Jr., Nicholas Scott - Obořilová, R. - Farka, Z. - Skládal, P. - Saláková, A. - Spasovová, Monika - Houska, Milan - Kalhotka, L. - Vaisocherová-Lísalová, Hana
A comparative assessment of a piezoelectric biosensor based on a new antifouling nanolayer and cultivation methods: Enhancing S. aureus detection in fresh dairy products.
Current Research in Biotechnology. Roč. 6, Jan. (2023), č. článku 100166. ISSN 2590-2628. E-ISSN 2590-2628
Grant CEP: GA ČR(CZ) GA21-19779S
Grant ostatní: AV ČR(CZ) LQ100101902
Program: Prémie Lumina quaeruntur
Institucionální podpora: RVO:68378271
Klíčová slova: food safety * biosensor * antifouling coating * quartz crystal microbalance * cultivation-based methods * detection of Staphylococcus aureus dairy products
Obor OECD: Biophysics
Impakt faktor: 3.6, rok: 2023
Způsob publikování: Open access
DOI: https://doi.org/10.1016/j.crbiot.2023.100166
Ensuring dairy product safety demands rapid and precise Staphylococcus aureus (S. aureus) detection. Biosensors show promise, but their performance is often demonstrated in model samples using non-native pathogens and has never been studied towards S. aureus detection in naturally contaminated samples. This study addresses the gap by directly comparing results taken with a novel piezoelectric biosensor, capable of one-step detection, with four conventional cultivation-based methods. Our findings reveal that this biosensor, based on an antifouling nanolayer-coated biochip, exhibits exceptional resistance to biofouling from unprocessed dairy products and is further capable of specific S. aureus detection. Notably, it performed comparably to Petrifilm and Baird-Parker methods but delivered results in only 30 min, bringing a substantial reduction from the 24 h required by cultivation-based techniques.
Trvalý link: https://hdl.handle.net/11104/0350252
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