0582101 - FGÚ 2025 RIV GB eng J - Článek v odborném periodiku
Blanquer, Andreu - Kuželová Košťáková, E. - Filová, Elena - Lisnenko, M. - Brož, Antonín - Müllerová, J. - Novotný, V. - Havlíčková, K. - Jakubková, Š. - Hauzerová, Š. - Heczková, B. - Procházková, R. - Bačáková, Lucie - Jenčová, V.
A novel bifunctional multilayered nanofibrous membrane combining polycaprolactone and poly (vinyl alcohol) enriched with platelet lysate for skin wound healing.
Nanoscale. Roč. 16, č. 4 (2024), s. 1924-1941. ISSN 2040-3364. E-ISSN 2040-3372
Grant CEP: GA MŠMT(CZ) LX22NPO5104; GA MŠMT(CZ) EH22_008/0004562; GA MŠMT(CZ) EF18_046/0016045
Výzkumná infrastruktura: Czech-BioImaging II - 90129
Institucionální podpora: RVO:67985823
Klíčová slova: electrospun scaffolds * aliphatic polyesters * rich plasma
Obor OECD: Biomaterials (as related to medical implants, devices, sensors)
Impakt faktor: 5.8, rok: 2023 ; AIS: 1.141, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://doi.org/10.1039/D3NR04705ADOI: https://doi.org/10.1039/d3nr04705a

Skin wound healing is a complex physiological process that involves various cell types, growth factors, cytokines, and other bioactive compounds. In this study, a novel dual-function multilayered nanofibrous membrane is developed for chronic wound application. The membrane is composed of five alternating layers of polycaprolactone (PCL) and poly (vinyl alcohol) (PVA) nanofibers (PCL-PVA) with a dual function: the PCL nanofibrous layers allow cell adhesion and growth, and the PVA layers enriched with incorporated platelet lysate (PCL-PVA + PL) serve as a drug delivery system for continuous release of bioactive compounds from PL into an aqueous environment. The material is produced using a needleless multi-jet electrospinning approach which can lead to homogeneous large-scale production. The bioactive PCL-PVA + PL membranes are cytocompatible and hemocompatible. A spatially compartmented co-culture of three cell types involved in wound healing keratinocytes, fibroblasts and endothelial cells is used for cytocompatibility studies. PCL-PVA + PL membranes enhance the proliferation of all cell types and increase the migration of both fibroblasts and endothelial cells. The membranes are also hemocompatible without any deleterious effect for thrombogenicity, hemolysis and coagulation. Thus, the beneficial effect of the PCL-PVA + PL membrane is demonstrated in vitro, making it a promising scaffold for the treatment of chronic wounds.
Trvalý link: https://hdl.handle.net/11104/0351227