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LEGO-lipophosphonoxins: length of hydrophobic module affects permeabilizing activity in target membranes of different phospholipid composition

  1. 1.
    0581956 - ÚOCHB 2025 RIV GB eng J - Článek v odborném periodiku
    Dugic, M. - Brzobohatá, H. - Mojr, Viktor - Dolejšová, T. - Lišková, P. - Do Pham, Duy Dinh - Rejman, Dominik - Mikušová, Gabriela - Fišer, R.
    LEGO-lipophosphonoxins: length of hydrophobic module affects permeabilizing activity in target membranes of different phospholipid composition.
    RSC Advances. Roč. 14, č. 4 (2024), s. 2745-2756. E-ISSN 2046-2069
    Grant CEP: GA ČR(CZ) GA22-08857S; GA MŠMT(CZ) LX22NPO5103
    Institucionální podpora: RVO:61388963
    Klíčová slova: lipophosphonoxin * LEGO-LPPO * antimicrobial
    Obor OECD: Organic chemistry
    Impakt faktor: 3.9, rok: 2022
    Způsob publikování: Open access
    https://doi.org/10.1039/D3RA07251G

    In the past few decades, society has faced rapid development and spreading of antimicrobial resistance due to antibiotic misuse and overuse and the immense adaptability of bacteria. Difficulties in obtaining effective antimicrobial molecules from natural sources challenged scientists to develop synthetic molecules with antimicrobial effect. We developed modular molecules named LEGO-Lipophosphonoxins (LEGO-LPPO) capable of inducing cytoplasmic membrane perforation. In this structure-activity relationship study we focused on the role of the LEGO-LPPO hydrophobic module directing the molecule insertion into the cytoplasmic membrane. We selected three LEGO-LPPO molecules named C9, C8 and C7 differing in the length of their hydrophobic chain and consisting of an alkenyl group containing one double bond. The molecule with the long hydrophobic chain (C9) was shown to be the most effective with the lowest MIC and highest perforation rate both in vivo and in vitro. We observed high antimicrobial activity against both G+ and G- bacteria with significant differences in LEGO-LPPOs mechanism of action on these two cell types. We observed a highly cooperative mechanism of LEGO-LPPO action on G- bacteria as well as on liposomes resembling G- bacteria. LEGO-LPPO action on G- bacteria was significantly slower compared to G+ bacteria suggesting the role of the outer membrane in affecting the LEGO-LPPOs perforation rate. This notion was supported by the higher sensitivity of the E. coli strain with a compromised outer membrane. Finally, we noted that the composition of the cytoplasmic membrane affects the activity of LEGO-LPPOs since the presence of phosphatidylethanolamine increases their membrane disrupting activity.
    Trvalý link: https://hdl.handle.net/11104/0350090

     
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