Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells

0581955 - ÚEM 2024 RIV CH eng J - Článek v odborném periodiku
Rössner ml., Pavel - Červená, Tereza - Echalar, Barbora - Palacká, Kateřina - Milcová, Alena - Novaková, Zuzana - Šíma, Michal - Šímová, Zuzana - Vaňková, Jolana - Holáň, Vladimír
Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells.
Toxics. Roč. 11, č. 3 (2023), č. článku 253. E-ISSN 2305-6304
Grant CEP: GA ČR(CZ) GA21-17720S; GA MŠMT(CZ) LM2018133; GA MŠMT(CZ) EF16_013/0001821; GA MŠMT(CZ) LM2018124
Institucionální podpora: RVO:68378041
Klíčová slova: metal nanoparticles * antimicrobial properties * mesenchymal stem cells * toxicity
Obor OECD: Public and environmental health
Impakt faktor: 4.6, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2305-6304/11/3/253

Some metal nanoparticles (NP) are characterized by antimicrobial properties with the potential to be used as alternative antibiotics. However, NP may negatively impact human organism, including mesenchymal stem cells (MSC), a cell population contributing to tissue growth and regeneration. To address these issues, we investigated the toxic effects of selected NP (Ag, ZnO, and CuO) in mouse MSC. MSC were treated with various doses of NP for 4 h, 24 h, and 48 h and multiple endpoints were analyzed. Reactive oxygen species were generated after 48 h CuO NP exposure. Lipid peroxidation was induced after 4 h and 24 h treatment, regardless of NP and/or tested dose. DNA fragmentation and oxidation induced by Ag NP showed dose responses for all the periods. For other NP, the effects were observed for shorter exposure times. The impact on the frequency of micronuclei was weak. All the tested NP increased the sensitivity of MSC to apoptosis. The cell cycle was most affected after 24 h, particularly for Ag NP treatment. In summary, the tested NP induced numerous adverse changes in MSC. These results should be taken into consideration when planning the use of NP in medical applications where MSC are involved.
Trvalý link: https://hdl.handle.net/11104/0350725