Počet záznamů: 1  

Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy

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    0580758 - MBÚ 2024 RIV CZ eng J - Článek v odborném periodiku
    Kaňa, Radek - Šedivá, Barbora - Prášil, Ondřej
    Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy.
    Photosynthetica. Roč. 61, Special issue (2023), s. 483-491. ISSN 0300-3604. E-ISSN 1573-9058
    Grant CEP: GA ČR(CZ) GA23-06593S; GA MŠMT(CZ) LO1416; GA MŠMT(CZ) ED2.1.00/19.0392
    Institucionální podpora: RVO:61388971
    Klíčová slova: Airyscan * cyanobacteria * frap * microdomain * photosystem * protein mobility * super-resolution microscopy * thylakoid membrane heterogeneity
    Obor OECD: Microbiology
    Impakt faktor: 2.1, rok: 2023
    Způsob publikování: Open access
    https://ps.ueb.cas.cz/artkey/phs-202304-0009_microdomains-heterogeneity-in-the-thylakoid-membrane-proteins-visualized-by-super-resolution-microscopy.php

    The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using superresolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes, countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions.
    Trvalý link: https://hdl.handle.net/11104/0349519

     
     
Počet záznamů: 1  

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