DISCOVERY OF NOVEL AVIAN INTERFERON REGULATORY FACTORS

0580686 - ÚMG 2024 RIV CZ eng A - Abstrakt
Ungrová, Lenka - Nehyba, Jiří - Krchlíková, Veronika - Hron, Tomáš - Hejnar, Jiří - Elleder, Daniel
DISCOVERY OF NOVEL AVIAN INTERFERON REGULATORY FACTORS.
Czech Chemical Society Symposium Series. Roč. 21, č. 5 (2023), č. článku P-61. ISSN 2336-7202.
[Annual meeting of the National Institute of Virology and Bacteriology (NIVB) /2./. 02.10.2023-05.10.2023, Kutná Hora]
Grant CEP: GA MŠMT(CZ) LX22NPO5103
Institucionální podpora: RVO:68378050
Klíčová slova: Interferon regulatory factors * RIG-I-like receptors * IFN-β
Obor OECD: Virology
http://ccsss.cz/index.php/ccsss/issue/view/41

Interferon regulatory factors (IRFs) are transcription factors that play a key role in anti-viral immunity response of vertebrates. After pathogen recognition by RIG-I-like receptors (RLR), IRF3 and IRF7 activate expression of IFN-β which is together with other type I interferons responsible for expression of interferon-stimulated genes (ISGs) through JAK-STAT1-IRF9 pathway1,2. These pathways are well described in mammals and some other vertebrates but there is little information available in birds. Moreover, IRF9 and IRF3 are thought to be missing in birds4,5even though both of the pathways are functional. Using in silicocomparative analysis and de novo assembly, we were able to annotate IRF9 in multiple avian species across most avian orders, and IRF3 in paleognath birds. We used duck fibroblast cell line as a model system for characterization of avian IRF9. For the first time, we show the role of duck IRF9 (dIRF9) in type I IFN pathway and its role in regulation of ISGs expression after interferon stimulation. Our results suggest time-dependent decrease in the induction of ISGs in the absence of dIRF9 that is partially restored in cells with stable expression of endogenous dIRF9 as well as in cells transiently transfected with endogenous dIRF9. We also focused on a general effect of the dIRF9 absence in cells with or without interferon stimulation using RNAseq data. In summary, we provide the first characterization of previously unknown avian IRF genes
Trvalý link: https://hdl.handle.net/11104/0349445