Efficacy of the Vaccine Candidate Based on the P0 Peptide against iDermacentor nitens/i and iIxodes ricinus/i Ticks

0580597 - BC 2024 RIV CH eng J - Článek v odborném periodiku
Rodriguez-Mallon, A. - Guzman, P.E.E. - Bello, Y. - Domingos, A. - Antunes, S. - Kopáček, Petr - Santos, A.S. - Velez, A. - Perner, Jan - Bravo, F.L.L. - Frantová, Helena - Erhart, Jan - Rodriguez, R. - Fuentes, A. - Diago, D. - Joglar, M. - Mendez, L. - Estrada, M.P.
Efficacy of the Vaccine Candidate Based on the P0 Peptide against iDermacentor nitens/i and iIxodes ricinus/i Ticks.
Pathogens. Roč. 12, č. 11 (2023), č. článku 1365. E-ISSN 2076-0817
Grant CEP: GA ČR(CZ) GA20-05736S
Institucionální podpora: RVO:60077344
Klíčová slova: ribosomal-protein p0 * dog tick * bm86 antigen * brown dog * acari * microplus * humans * ticks * P0 protein * anti-tick vaccine * tick control * vaccination
Obor OECD: Immunology
Impakt faktor: 3.7, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2076-0817/12/11/1365

The control of ticks through vaccination offers a sustainable alternative to the use of chemicals that cause contamination and the selection of resistant tick strains. However, only a limited number of anti-tick vaccines have reached commercial realization. In this sense, an antigen effective against different tick species is a desirable target for developing such vaccines. A peptide derived from the tick P0 protein (pP0) conjugated to a carrier protein has been demonstrated to be effective against the Rhipicephalus microplus, Rhipicephalus sanguineus, and Amblyomma mixtum tick species. The aim of this work was to assess the efficacy of this peptide when conjugated to the Bm86 protein against Dermacentor nitens and Ixodes ricinus ticks. An RNAi experiment using P0 dsRNA from I. ricinus showed a dramatic reduction in the feeding of injected female ticks on guinea pigs. In the follow-up vaccination experiments, rabbits were immunized with the pP0-Bm86 conjugate and challenged simultaneously with larvae, nymphs, and the adults of I. ricinus ticks. In the same way, horses were immunized with the pP0-Bm86 conjugate and challenged with D. nitens larva. The pP0-Bm86 conjugate showed efficacies of 63% and 55% against I. ricinus and D. nitens ticks, respectively. These results, combined with previous reports of efficacy for this conjugate, show the promising potential for its development as a broad-spectrum anti-tick vaccine.
Trvalý link: https://hdl.handle.net/11104/0349360