Circadian rhythms and circadian clock gene homologs of complex alga iChromera velia/i

0580594 - BC 2024 RIV CH eng J - Článek v odborném periodiku
Richtová, Jitka - Bazalova, O. - Horák, Aleš - Tomčala, A. - Gonepogu, V.G. - Oborník, Miroslav - Doležel, David
Circadian rhythms and circadian clock gene homologs of complex alga iChromera velia/i.
Frontiers in Plant Science. Roč. 14, DEC (2023), č. článku 1226027. ISSN 1664-462X. E-ISSN 1664-462X
Grant CEP: GA ČR(CZ) GA21-03224S; GA MŠMT(CZ) EF16_019/0000759; GA ČR(CZ) GA22-10088S; GA ČR(CZ) GA23-06203S
Institucionální podpora: RVO:60077344
Klíčová slova: blue-light * ectocarpus phaeophyta * fast responses * protein * photosynthesis * photoreceptors * identification * localization * cryptochrome * mechanisms * apicomplexa * Chromera velia * circadian clock * cryptochrome * zoospore formation
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.6, rok: 2022
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fpls.2023.1226027/full

Most organisms on Earth are affected by periodic changes in their environment. The circadian clock is an endogenous device that synchronizes behavior, physiology, or biochemical processes to an approximately 24-hour cycle, allowing organisms to anticipate the periodic changes of day and night. Although circadian clocks are widespread in organisms, the actual molecular components differ remarkably among the clocks of plants, animals, fungi, and prokaryotes. Chromera velia is the closest known photosynthetic relative of apicomplexan parasites. Formation of its motile stage, zoospores, has been described as associated with the light part of the day. We examined the effects on the periodic release of the zoospores under different light conditions and investigated the influence of the spectral composition on zoosporogenesis. We performed a genomic search for homologs of known circadian clock genes. Our results demonstrate the presence of an almost 24-hour free-running cycle of zoosporogenesis. We also identified the blue light spectra as the essential compound for zoosporogenesis. Further, we developed a new and effective method for zoospore separation from the culture and estimated the average motility speed and lifespan of the C. velia zoospores. Our genomic search identified six cryptochrome-like genes, two genes possibly related to Arabidopsis thaliana CCA/LHY, whereas no homolog of an animal, cyanobacterial, or fungal circadian clock gene was found. Our results suggest that C. velia has a functional circadian clock, probably based mainly on a yet undefined mechanism.
Trvalý link: https://hdl.handle.net/11104/0349358