σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription

0580571 - MBÚ 2025 RIV GB eng J - Článek v odborném periodiku
Pospíšil, Jiří - Schwarz, Marek - Ziková, Alice - Vítovská, Dragana - Hradilová, Miluše - Kolář, Michal - Křenková, Alena - Hubálek, Martin - Krásný, Libor - Vohradský, Jiří
σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription.
Communications Biology. Roč. 7, January 6 (2024), č. článku 46. E-ISSN 2399-3642
Grant CEP: GA ČR(CZ) GF22-06342K; GA MŠMT(CZ) LM2023055; GA MŠMT(CZ) LX22NPO5103
Výzkumná infrastruktura: ELIXIR CZ III - 90255
Institucionální podpora: RVO:61388971 ; RVO:68378050 ; RVO:61388963
Klíčová slova: σ factors * proteins * Streptomyces coelicolor * gene expression
Obor OECD: Microbiology; Biochemistry and molecular biology (UMG-J)
Impakt faktor: 5.9, rok: 2022
Způsob publikování: Open access
https://www.nature.com/articles/s42003-023-05716-y

σ factors are considered as positive regulators of gene expression. Here we reveal the opposite, inhibitory role of these proteins. We used a combination of molecular biology methods and computational modeling to analyze the regulatory activity of the extracytoplasmic σE factor from Streptomyces coelicolor. The direct activator/repressor function of σE was then explored by experimental analysis of selected promoter regions in vivo. Additionally, the σE interactome was defined. Taken together, the results characterize σE, its regulation, regulon, and suggest its direct inhibitory function (as a repressor) in gene expression, a phenomenon that may be common also to other σ factors and organisms.
Trvalý link: https://hdl.handle.net/11104/0349344