First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics

0580535 - ÚBO 2025 RIV NL eng J - Článek v odborném periodiku
Pafčo, Barbora - Nosková, E. - Ilík, V. - Anettová, L. - Červená, Barbora - Kreisinger, J. - Pšenková, I. - Václavek, P. - Vyhlídalová, T. - Ježková, J. - Malát, K. - Mihalca, A. D. - Modrý, David
First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics.
Veterinary Parasitology: Regional Studies and Reports. Roč. 47, January (2024), č. článku 100961. E-ISSN 2405-9390
Výzkumná infrastruktura: NCMG II - 90132; e-INFRA CZ - 90140
Institucionální podpora: RVO:68081766 ; RVO:60077344
Klíčová slova: Anthelmintic treatment * Cattle * Resistance * Strongylid nematodes
Obor OECD: Veterinary science
Impakt faktor: 1.4, rok: 2022
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S2405939023001314?via%3Dihub

Parasitic diseases and mitigation of their effects play an important role in the health management of grazing livestock worldwide, with gastrointestinal strongylid nematodes being of prominent importance. These helminths typically occur in complex communities, often composed of species from numerous strongylid genera. Detecting the full diversity of strongylid species in non-invasively collected faecal samples is nearly impossible using conventional methods. In contrast, high-throughput amplicon sequencing (HTS) can effectively identify co-occurring species. During the four-year project, we collected and analysed faecal samples from beef cattle on >120 farms throughout the Czech Republic. Strongylids were the predominant nematodes, detected in 56% of the samples, but at a low level of infection. The apparent limitations in identifying strongylid taxa prompted this pilot study on a representative group of samples testing positive for strongylids using ITS-2 metabarcoding. The most widespread genera parasitizing Czech cattle were Ostertagia (O. ostertagi) and Oesophagostomum spp., followed by Trichostrongylus and Cooperia, while Bunostomum, Nematodirus and Chabertia were present only in a minority. As comparative material, 21 samples of cattle from the Danube Delta in Romania were used, which, in contrast, were dominated by Haemonchus placei. Finally, the effect of ivermectin treatment was tested at two Czech farms. After treatment with the anthelmintic, there was a shift in the strongylid communities, with a dominance of Cooperia and Ostertagia.
Trvalý link: https://hdl.handle.net/11104/0349298