Počet záznamů: 1  

Regulation of phosphosignaling pathways involved in transcription of cell cycle target genes by TRH receptor activation in GH1 cells

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    0580215 - FGÚ 2024 RIV NL eng J - Článek v odborném periodiku
    Drastichová, Z. - Trubačová, Radka - Novotný, J.
    Regulation of phosphosignaling pathways involved in transcription of cell cycle target genes by TRH receptor activation in GH1 cells.
    Biomedicine & Pharmacotherapy. Roč. 168, Dec (2023), č. článku 115830. ISSN 0753-3322. E-ISSN 1950-6007
    Institucionální podpora: RVO:67985823
    Klíčová slova: β-arrestin2 * cell cycle * phosphosignaling * Taltirelin * Thyrotropin-releasing hormone
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 7.5, rok: 2022
    Způsob publikování: Open access
    https://doi.org/10.1016/j.biopha.2023.115830

    Thyrotropin-releasing hormone (TRH) is known to activate several cellular signaling pathway, but the activation of the TRH receptor (TRH-R) has not been reported to regulate gene transcription. The aim of this study was to identify phosphosignaling pathways and phosphoprotein complexes associated with gene transcription in GH1 pituitary cells treated with TRH or its analog, taltirelin (TAL), using label-free bottom-up mass spectrometry-based proteomics. Our detailed analysis provided insight into the mechanism through which TRH-R activation may regulate the transcription of genes related to the cell cycle and proliferation. It involves control of the signaling pathways for β-catenin/Tcf, Notch/RBPJ, p53/p21/Rbl2/E2F, Myc, and YY1/Rb1/E2F through phosphorylation and dephosphorylation of their key components. In many instances, the phosphorylation patterns of differentially phosphorylated phosphoproteins in TRH- or TAL-treated cells were identical or displayed a similar trend in phosphorylation. However, some phosphoproteins, especially components of the Wnt/β-catenin/Tcf and YY1/Rb1/E2F pathways, exhibited different phosphorylation patterns in TRH- and TAL-treated cells. This supports the notion that TRH and TAL may act, at least in part, as biased agonists. Additionally, the deficiency of β-arrestin2 resulted in a reduced number of alterations in phosphorylation, highlighting the critical role of β-arrestin2 in the signal transduction from TRH-R in the plasma membrane to transcription factors in the nucleus.
    Trvalý link: https://hdl.handle.net/11104/0348967

     
     
Počet záznamů: 1  

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