Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures

0580038 - ÚIACH 2024 RIV GB eng J - Článek v odborném periodiku
Killinger, Michael - Kratochvílová, Adéla - Ingeborg Reihs, E. - Matalová, Eva - Klepárník, Karel - Rothbauer, M.
Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures.
Journal of Biological Engineering. Roč. 17, č. 1 (2023), č. článku 77. ISSN 1754-1611. E-ISSN 1754-1611
Grant CEP: GA ČR(CZ) GA20-00726S
Institucionální podpora: RVO:68081715 ; RVO:67985904
Klíčová slova: bone on a chip * microwells * micropillars
Obor OECD: Analytical chemistry; Cell biology (UZFG-Y)
Impakt faktor: 5.6, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1186/s13036-023-00395-z

In this study, we consequently developed, optimized and characterized an advanced perfused microfluidic platform to improve the reliability of 3D bone cell cultivation and to enhance aspects of bone tissue maturation in vitro. Moreover, biomechanical stimulation generated by fluid flow inside the arrayed chamber, was used to mimic a more dynamic cell environment emulating a highly vascularized bone we expected to improve the osteogenic 3D microenvironment in the developed multifunctional spheroid-array platform.
Trvalý link: https://hdl.handle.net/11104/0348820