Počet záznamů: 1
The opportunistic protist, iGiardia intestinalis/i, occurs in gut-healthy humans in a high-income country
- 1.0579876 - BC 2024 RIV GB eng J - Článek v odborném periodiku
Brožová, Kristýna - Jirků, Milan - Lhotská, Zuzana - Květoňová, Dana - Kadlecová, Oldřiška - Stensvold, C.R. - Samaš, P. - Petrželková, Klára Judita - Jirků-Pomajbíková, Kateřina
The opportunistic protist, iGiardia intestinalis/i, occurs in gut-healthy humans in a high-income country.
Emerging Microbes & Infections. Roč. 12, č. 2 (2023), č. článku 2270077. ISSN 2222-1751. E-ISSN 2222-1751
Grant CEP: GA ČR(CZ) GA22-04837S
Institucionální podpora: RVO:60077344
Klíčová slova: molecular epidemiology * entamoeba-histolytica * duodenalis * prevalence * lamblia * gene * Giardia intestinalis, human volunteers * qPCR * conventional-PCR * quantification * contact with animals
Obor OECD: Parasitology
Impakt faktor: 8.4, rok: 2023
Způsob publikování: Open access
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2270077
Giardia intestinalis, a cosmopolitan gastrointestinal protist, is detected mainly in patients with clinical giardiasis in high-income countries. In contrast, there is very little information on the presence of Giardia in asymptomatic individuals. Therefore, the aim of this study was to determine the presence and prevalence of Giardia in gut-healthy volunteers in the Czech Republic and to perform a comparative evaluation of different diagnostic methods, since Giardia diagnostics is complicated. Our results confirmed that the qPCR method is the most sensitive method for detecting Giardia and revealed a prevalence of 7% (22/296) in asymptomatic individuals. In most cases, the colonization intensity ranged from 10(-1)-10(1). A conventional PCR protocol targeting the TPI gene was used to identify the assemblages. However, this protocol had limited sensitivity for Giardia amplification, effectively detecting colonization above an intensity of 104. In addition, Giardia was detected in 19% of the animals, which were closely associated with the study participants. However, due to methodological limitations, zoonotic transmission could not be clearly confirmed. Notably, contact with animals proved to be the only factor that had a significant impact on the incidence of Giardia in gut-healthy humans.
Trvalý link: https://hdl.handle.net/11104/0348660
Počet záznamů: 1