The dynamics of telomere length in primary and metastatic colorectal cancer lesions

0579640 - ÚEM 2024 RIV US eng J - Článek v odborném periodiku
Kroupa, Michal - Kubeček, O. - Tomášová, Kristýna - Hanák, Petr - Krupová, M. - Červená, Klára - Šišková, Anna - Rosendorf, Jáchym - Hošek, P. - Vodičková, Ludmila - Vodička, Pavel - Liška, Václav - John, S. - Vymetálková, Veronika - Petera, J.
The dynamics of telomere length in primary and metastatic colorectal cancer lesions.
Scientific Reports. Roč. 13, č. 1 (2023), č. článku 9097. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA MŠMT LX22NPO5102; GA MZd(CZ) NV19-09-00237; GA MZd(CZ) NU22J-03-00028; GA ČR(CZ) GA21-27902S
Institucionální podpora: RVO:68378041
Klíčová slova: breast-cancer * pathological features * instability * prognosis * colon
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.6, rok: 2022
Způsob publikování: Open access
https://www.nature.com/articles/s41598-023-35835-9

Telomeric sequences, the structures comprised of hexanucleotide repeats and associated proteins, play a pivotal role in chromosome end protection and preservation of genomic stability. Herein we address telomere length (TL) dynamics in primary colorectal cancer (CRC) tumour tissues and corresponding liver metastases. TL was measured by multiplex monochrome real- time qPCR in paired samples of primary tumours and liver metastases along with non-cancerous reference tissues obtained from 51 patients diagnosed with metastatic CRC. Telomere shortening was observed in the majority of primary tumour tissues compared to non-cancerous mucosa (84.1%, p < 0.0001). Tumours located within the proximal colon had shorter TL than those in the rectum (p < 0.05). TL in liver metastases was not significantly different from that in primary tumours (p = 0.41). TL in metastatic tissue was shorter in the patients diagnosed with metachronous liver metastases than in those diagnosed with synchronous liver metastases (p = 0.03). The metastatic liver lesions size correlated with the TL in metastases (p < 0.05). Following the neoadjuvant treatment, the patients with rectal cancer had shortened telomeres in tumour tissue than prior to the therapy (p = 0.01). Patients with a TL ratio between tumour tissue and the adjacent non-cancerous mucosa of = 0.387 were associated with increased overall survival (p = 0.01). This study provides insights into TL dynamics during progression of the disease. The results show TL differences in metastatic lesions and may help in clinical practice to predict the patient's prognosis.
Trvalý link: https://hdl.handle.net/11104/0348447