Top-Down Proteoform Analysis by 2D MS with Quadrupolar Detection

0579428 - MBÚ 2024 RIV US eng J - Článek v odborném periodiku
Polák, Marek - Palasser, M. - Kádek, Alan - Kavan, Daniel - Wootton, C. A. - Delsuc, M. - Breuker, K. - Novák, Petr - van Agthoven, Maria Andrea
Top-Down Proteoform Analysis by 2D MS with Quadrupolar Detection.
Analytical Chemistry. Roč. 95, č. 44 (2023), s. 16123-16130. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA MŠMT(CZ) EF18_053/0017705; GA ČR(CZ) GA22-27695S; GA MŠMT(CZ) ED1.1.00/02.0109
GRANT EU: European Commission(XE) 731077 - EU_FT-ICR_MS
Institucionální podpora: RVO:61388971
Klíčová slova: 2-dimensional mass-spectrometry * electron-capture dissociation * chemical cross-linking * ultraviolet photodissociation * resolution * precursor
Obor OECD: Analytical chemistry
Impakt faktor: 7.4, rok: 2022
Způsob publikování: Open access
https://pubs.acs.org/doi/10.1021/acs.analchem.3c02225

Two-dimensional mass spectrometry (2D MS) is a multiplexed tandem mass spectrometry method that does not rely on ion isolation to correlate the precursor and fragment ions. On a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS), 2D MS instead uses the modulation of precursor ion radii inside the ICR cell before fragmentation and yields 2D mass spectra that show the fragmentation patterns of all the analytes. In this study, we perform 2D MS for the first time with quadrupolar detection in a dynamically harmonized ICR cell. We discuss the advantages of quadrupolar detection in 2D MS and how we adapted existing data processing techniques for accurate frequency-to-mass conversion. We apply 2D MS with quadrupolar detection to the top-down analysis of covalently labeled ubiquitin with ECD fragmentation, and we develop a workflow for label-free relative quantification of biomolecule isoforms in 2D MS.
Trvalý link: https://hdl.handle.net/11104/0348240