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Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution
- 1.0579344 - ÚEB 2024 RIV US eng J - Článek v odborném periodiku
Skalický, Vladimír - Antoniadi, I. - Pěnčík, Aleš - Chamrád, Ivo - Lenobel, René - Kubeš, Martin - Zatloukal, M. - Žukauskaitė, A. - Strnad, Miroslav - Ljung, K. - Novák, Ondřej
Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution.
Plant Journal. Roč. 116, č. 6 (2023), s. 1825-1841. ISSN 0960-7412. E-ISSN 1365-313X
Grant CEP: GA ČR GA20-22875S
Institucionální podpora: RVO:61389030
Klíčová slova: Arabidopsis thaliana * Auxin * cytokinin * flow cytometry * subcellular fractionation * subcellular homeostasis * technical advances
Obor OECD: Plant sciences, botany
Impakt faktor: 7.2, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1111/tpj.16456
Auxins and cytokinins are two major families of phytohormones that control most aspects of plant growth, development and plasticity. Their distribution in plants has been described, but the importance of cell- and subcellular-type specific phytohormone homeostasis remains undefined. Herein, we revealed auxin and cytokinin distribution maps showing their different organelle-specific allocations within the Arabidopsis plant cell. To do so, we have developed Fluorescence-Activated multi-Organelle Sorting (FAmOS), an innovative subcellular fractionation technique based on flow cytometric principles. FAmOS allows the simultaneous sorting of four differently labelled organelles based on their individual light scatter and fluorescence parameters while ensuring hormone metabolic stability. Our data showed different subcellular distribution of auxin and cytokinins, revealing the formation of phytohormone gradients that have been suggested by the subcellular localization of auxin and cytokinin transporters, receptors and metabolic enzymes. Both hormones showed enrichment in vacuoles, while cytokinins were also accumulated in the endoplasmic reticulum.
Trvalý link: https://hdl.handle.net/11104/0348182
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