The mechanism of immune dysregulation caused by porcine reproductive and respiratory syndrome virus (PRRSV)

0578827 - MBÚ 2024 RIV NL eng J - Článek v odborném periodiku
Šinkora, Marek - Toman, M. - Štěpánová, Kateřina - Štěpánová, H. - Levá, L. - Šinkorová, Jana - Moutelíková, R. - Salát, J. - Šrůtková, Dagmar - Schwarzer, Martin - Šinkora, Šimon - Skalníková, Helena - Nechvátalová, K. - Hudcovic, Tomáš - Hermanová, Petra - Pfeiferová, Šárka - Kratochvílová, Mirka - Kavanová, L. - Dušánková, Blanka - Šinkora jr., Marek
The mechanism of immune dysregulation caused by porcine reproductive and respiratory syndrome virus (PRRSV).
Microbes and Infection. Roč. 25, č. 7 (2023), č. článku 105146. ISSN 1286-4579. E-ISSN 1769-714X
Grant CEP: GA ČR(CZ) GA20-03282S
Institucionální podpora: RVO:61388971 ; RVO:67985904
Klíčová slova: antibody repertoire development * b-cell lymphogenesis * delta t-lymphocytes * neonatal piglets * thymocytes * infection * subsets * thymus * fetal * swine * Porcine respiratory and reproductive * syndrome virus * Thymocytes * Tcell precursors * Lymhocytes * Animals
Obor OECD: Infectious Diseases; Biochemistry and molecular biology (UZFG-Y)
Impakt faktor: 5.8, rok: 2022
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S1286457923000497?via%3Dihub

PRRSV is capable of evading the effective immune response, thus persisting in piglets and throughout the swine herd. We show here that PRRSV invades the thymus and causes depletion of T-cell precursors and alteration of the TCR repertoire. Developing thymocytes are affected during negative selection when they transit from the triple-negative to triple-positive stages at the corticomedullary junction just before entering the medulla. The restriction of repertoire diversification occurs in both helper and cytotoxic abT cells. As a result, critical viral epitopes are tolerated, and infection becomes chronic. However, not all viral epitopes are tolerated. Infected piglets develop antibodies capable of recognizing PRRSV, but these are not virus neutralizing. Further analysis showed that the lack of an effective immune response against the critical viral structures results in the absence of a germinal center response, overactivation of T and B cells in the periphery, robust production of useless antibodies of all isotypes, and the inability to eliminate the virus. Overall, the results show how a respiratory virus that primarily infects and destroys myelomonocytic cells has evolved strategies to disrupt the immune system. These mechanisms may be a prototype for how other viruses can similarly modulate the host immune system.
Trvalý link: https://hdl.handle.net/11104/0347742