Characterization of the miniaturized platform for cell migration studies

0577687 - ÚIACH 2024 CZ eng A - Abstrakt
Lišková, Petra - Jedličková, Adriena - Killinger, Michael - Buchtová, Marcela - Václavek, Tomáš
Characterization of the miniaturized platform for cell migration studies.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 64-64. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
Institucionální podpora: RVO:68081715 ; RVO:67985904
Klíčová slova: micropillars * miniaturization * cell migration
Obor OECD: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

This work aims to design and fabricate a polydimethylsiloxane (PDMS) microfluidic cell-culture platform for testing natural cell mobility. The main feature is a circular arrangement of micropillars in a structure that forms an artificial barrier. The dimensions of micropillars and gaps can be easily adjusted in our design, and typically multiple devices are produced in one casting step, as the resulting PDMS cast is cut into individual cell-migration devices. Multiple cell-migration devices are then bonded to a single Petri dish so it serves as a powerful model for characterising malignant cells by label-free mechano-sorting. This platform will be used to test the ability of different tumour cells to change their shape and squeeze across the gaps between the pillars. In addition, the platform will be used for co-culture experiments with different populations of cells separated by micropillars or extracellular gels.
Trvalý link: https://hdl.handle.net/11104/0346813