Differentiation of Biofilm-Positive and Biofilm-Negative Candida Parapsilosis Strains by Capillary Isoelectric Focusing

0577677 - ÚIACH 2024 CZ eng A - Abstrakt
Kubesová, Anna - Šalplachta, Jiří - Růžička, F. - Holá, C.
Differentiation of Biofilm-Positive and Biofilm-Negative Candida Parapsilosis Strains by Capillary Isoelectric Focusing.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 63-63. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
Grant CEP: GA MZd(CZ) NU22-05-00110
Institucionální podpora: RVO:68081715
Klíčová slova: Candida parapsilosis * capillary isoelectric focusation * pI marker
Obor OECD: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

One of the opportunistic pathogens that can cause life-threatening infections is Candida parapsilosis. It may colonize both natural and artificial surfaces in the host's body because of the production of a robust, adhesive biofilm layer. Patients with candidemia have considerably greater rates of mortality when exposed to biofilm-positive strains of Candida parapsilosis than when exposed to biofilm-negative strains. Techniques used to determine the ability to form biofilms are usually based on growth on suitable culture surfaces or microtiter plates. These procedures take a long time and a change in the cultural environment can easily affect the results. As a different approach, the physico-chemical characteristics of the cell surface, particularly in a cell surface charge, can be used to differentiate between biofilm-positive and biofilm-negative Candida parapsilosis strains. Determination of isoelectric points of Candida parapsilosis strains by capillary isoelectric focusing seems to be a useful technique to distinguish whether or not Candida parapsilosis strains form biofilm. This determination process is very fast taking a few minutes. The calculation and determination of isoelectric points was achieved by comparing the migration times of the tested yeasts and pI markers.
Trvalý link: https://hdl.handle.net/11104/0346807