Optimization of glycoproteomic protocol for efficient and sensitive analysis of N-Linked glycans by CE/LIF

0577620 - ÚIACH 2024 CZ eng A - Abstrakt
Bobálová, Janette - Smolková, Denisa - Strouhalová, Dana - Čmelík, Richard - Lavická, Jana
Optimization of glycoproteomic protocol for efficient and sensitive analysis of N-Linked glycans by CE/LIF.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 44-44. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
Grant CEP: GA ČR(CZ) GA22-00236S
Institucionální podpora: RVO:68081715
Klíčová slova: CE/LIF analysis * cascade blue hydrazide * glycans
Obor OECD: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

In this work, we report the effect of different proteomic protocols varying in enzyme or digestion procedures followed by derivatization by Cascade Blue hydrazide (8-(2-hydrazino-2-oxoethoxy)pyrene-1,3,6-trisulfonic acid trisodium salt, CBH) on the glycan detection in CE/LIF analysis. CBH represents a more reactive analog of APTS widely used for glycan labeling before CE analysis [1]. Ribonuclease B was selected as a model glycoprotein and four digestion protocols (alkylation, in gel/in solution treatment, purification step, etc.) were applied in order to obtain highly sensitive detection of labeled glycans by CE/LIF analysis.
Trvalý link: https://hdl.handle.net/11104/0346743