A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition

0577615 - ÚIACH 2024 CZ eng A - Abstrakt
Melicherová, Natália - Gloser, V. - Skalák, J. - Trtílek, M. - Lavická, Jana - Foret, František … celkem 7 autorů
A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 37-37. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
Grant CEP: GA MŠMT EF16_026/0008446
Institucionální podpora: RVO:68081715
Klíčová slova: capillary electrophoresis * plant sap * sampling * stress * xylem
Obor OECD: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

In this work, an online sampling of plants combined with an efficient capillary electrophoresis (CE)-based method was developed and applied to study the kinetics of changes in the xylem sap composition and to assess plant fitness under stress conditions comprehensively [1,2]. A laboratory-built CE device was developed to provide online sampling and CE analysis of various ionogenic species in the plant sap during plant stress response. The short CE analysis time allows for capturing time-dependent kinetic changes in the living plant during the stress response. The developed device successfully monitored chloride, nitrate, and sulfate ions in the plant xylem during the salt stress or stress caused by nitrate deficiency.
Trvalý link: https://hdl.handle.net/11104/0346741