Astrocyte-like subpopulation of NG2 glia in the adult mouse cortex exhibits characteristics of neural progenitor cells

0576841 - ÚMG 2025 RIV US eng J - Článek v odborném periodiku
Janečková, Lucie - Knotek, Tomáš - Kriška, Ján - Heřmanová, Zuzana - Kirdajová, Denisa - Kubovčiak, Jan - Berková, Linda - Turečková, Jana - Garcia, Camacho, Sara - Galušková, Kateřina - Kolář, Michal - Anděrová, Miroslava - Kořínek, Vladimír
Astrocyte-like subpopulation of NG2 glia in the adult mouse cortex exhibits characteristics of neural progenitor cells.
Glia. Roč. 72, č. 2 (2024), s. 245-273. ISSN 0894-1491. E-ISSN 1098-1136
Grant CEP: GA ČR(CZ) GA21-24674S; GA MŠMT(CZ) LM2018131; GA MŠMT(CZ) LM2023050; GA MŠMT(CZ) EF18_046/0016045
Institucionální podpora: RVO:68378050 ; RVO:68378041
Klíčová slova: chondroitin sulfate proteoglycan 4 * focal cerebral ischemia * neurogenesis * NG2 glia heterogeneity * oligodendrocyte precursor cell * single-cell RNA sequencing
Obor OECD: Cell biology; Neurosciences (including psychophysiology (UEM-P)
Impakt faktor: 6.2, rok: 2022
Způsob publikování: Open access
https://onlinelibrary.wiley.com/doi/10.1002/glia.24471

Glial cells expressing neuron-glial antigen 2 (NG2), also known as oligodendrocyte progenitor cells (OPCs), play a critical role in maintaining brain health. However, their ability to differentiate after ischemic injury is poorly understood. The aim of this study was to investigate the properties and functions of NG2 glia in the ischemic brain. Using transgenic mice, we selectively labeled NG2-expressing cells and their progeny in both healthy brain and after focal cerebral ischemia (FCI). Using single-cell RNA sequencing, we classified the labeled glial cells into five distinct subpopulations based on their gene expression patterns. Additionally, we examined the membrane properties of these cells using the patch-clamp technique. Of the identified subpopulations, three were identified as OPCs, whereas the fourth subpopulation had characteristics indicative of cells likely to develop into oligodendrocytes. The fifth subpopulation of NG2 glia showed astrocytic markers and had similarities to neural progenitor cells. Interestingly, this subpopulation was present in both healthy and post-ischemic tissue, however, its gene expression profile changed after ischemia, with increased numbers of genes related to neurogenesis. Immunohistochemical analysis confirmed the temporal expression of neurogenic genes and showed an increased presence of NG2 cells positive for Purkinje cell protein-4 at the periphery of the ischemic lesion 12 days after FCI, as well as NeuN-positive NG2 cells 28 and 60 days after injury. These results suggest the potential development of neuron-like cells arising from NG2 glia in the ischemic tissue. Our study provides insights into the plasticity of NG2 glia and their capacity for neurogenesis after stroke.
Trvalý link: https://hdl.handle.net/11104/0348332