Characterization of vestibular schwannoma tissues using liquid chromatography-tandem mass spectrometry analysis of specific peptide fragments separated by in-sample tryptic protein digestion followed by mathematical analysis

0576187 - ÚOCHB 2024 RIV US eng J - Článek v odborném periodiku
Tesařová, M. - Boušková, T. - Cejnar, P. - Šantrůček, J. - Peterková, L. - Fík, Z. - Sázelová, Petra - Kašička, Václav - Hynek, R.
Characterization of vestibular schwannoma tissues using liquid chromatography-tandem mass spectrometry analysis of specific peptide fragments separated by in-sample tryptic protein digestion followed by mathematical analysis.
Journal of Separation Science. Roč. 46, č. 22 (2023), č. článku 2300543. ISSN 1615-9306. E-ISSN 1615-9314
Institucionální podpora: RVO:61388963
Klíčová slova: in-sample protein digestion * liquid chromatography * principal component analysis * tandem mass spectrometry * vestibular schwannomas
Obor OECD: Analytical chemistry
Impakt faktor: 3.1, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1002/jssc.202300543

Vestibular schwannoma is the most common benign neoplasm of the cerebellopontine angle. Its first symptoms include hearing loss, tinnitus, and vestibular symptoms, followed by cerebellar and brainstem symptoms, along with palsy of the adjacent cranial nerves. However, the clinical picture has unpredictable dynamics and currently, there are no reliable predictors of tumor behavior. Hence, it is desirable to have a fast routine method for analysis of vestibular schwannoma tissues at the molecular level. The major objective of this study was to verify whether a technique using in-sample specific protein digestion with trypsin would have the potential to provide a proteomic characterization of these pathological tissues. The achieved results showed that the use of this approach with subsequent liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of released peptides allowed a fast identification of a considerable number of proteins in two differential parts of vestibular schwannoma tissue as well as in tissues of control healthy samples. Furthermore, mathematical analysis of MS data was able to discriminate between pathological vestibular schwannoma tissues and healthy tissues. Thus, in-sample protein digestion combined with LC-MS/MS separation and identification of released specific peptides followed by mathematical analysis appears to have the potential for routine characterization of vestibular schwannomas at the molecular level. Data are available via ProteomeXchange with identifier PXD045261.
Trvalý link: https://hdl.handle.net/11104/0345778


Vědecká data: PRIDE