Scalable production of tissue-like vascularized liver organoids from human PSCs

0576064 - FZÚ 2024 RIV KR eng J - Článek v odborném periodiku
Harrison, S.P. - Siller, R. - Yoshiaki, T. - Lunov, Oleg - Dejneka, Alexandr … celkem 36 autorů
Scalable production of tissue-like vascularized liver organoids from human PSCs.
Experimental and Molecular Medicine. Roč. 55, č. 9 (2023), s. 2005-2024. ISSN 1226-3613. E-ISSN 2092-6413
Institucionální podpora: RVO:68378271
Klíčová slova: organoids * drug toxicity * microscopy * single-cell RNA sequencing
Obor OECD: Biophysics
Impakt faktor: 12.8, rok: 2022
Způsob publikování: Open access

The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissues, including the liver. Current organoid protocols are characterized by a reliance on extracellular matrices (ECMs), patterning in 2D culture, costly growth factors and a lack of cellular diversity, structure, and organization. Current hepatic organoid models are generally simplistic and composed of hepatocytes or cholangiocytes. We have developed an approach that does not require 2D patterning, is ECM independent, and employs small molecules to mimic embryonic liver development that produces large quantities of liver-like organoids. The organoids exhibit key liver functions, including drug metabolism, serum protein production, urea synthesis and coagulation factor production, with preserved post-translational modifications such as N-glycosylation and functionality.
Trvalý link: https://hdl.handle.net/11104/0346848