Počet záznamů: 1  

Development of a novel genetic sexing strain of Ceratitis capitata based on an X autosome translocation

  1. 1.
    0575923 - BC 2024 RIV DE eng J - Článek v odborném periodiku
    Cáceres, C. - Bourtzis, K. - Gouvi, G. - Vreysen, M. J. B. - Somda, N. S. B. - Hejníčková, Martina - Marec, František - Meza, J. S.
    Development of a novel genetic sexing strain of Ceratitis capitata based on an X autosome translocation.
    Scientific Reports. Roč. 13, č. 1 (2023), č. článku 16167. ISSN 2045-2322. E-ISSN 2045-2322
    Institucionální podpora: RVO:60077344
    Klíčová slova: sterile insect technique * genetic sexing strain * Mediterranean fruit fly
    Obor OECD: Genetics and heredity (medical genetics to be 3)
    Impakt faktor: 4.6, rok: 2022
    Způsob publikování: Open access
    https://www.nature.com/articles/s41598-023-43164-0.pdf

    Genetic sexing strains (GSS), such as the Ceratitis capitata (medfly) VIENNA 8 strain, facilitate male-only releases and improve the efficiency and cost-effectiveness of sterile insect technique (SIT) applications. Laboratory domestication may reduce their genetic diversity and mating behaviour and hence, refreshment with wild genetic material is frequently needed. As wild males do not carry the T(Y,A) translocation, and wild females do not easily conform to artificial oviposition, the genetic refreshment of this GSS is a challenging and time-consuming process. In the present study, we report the development of a novel medfly GSS, which is based on a viable homozygous T(XX,AA) translocation using the same selectable markers, the white pupae and temperature-sensitive lethal genes. This allows the en masse cross of T(XX,AA) females with wild males, and the backcrossing of F1 males with the T(XX,AA) females thus facilitating the re-establishment of the GSS as well as its genetic refreshment. The rearing efficiency and mating competitiveness of the novel GSS are similar to those of the T(Y,A)-based VIENNA 8 GSS. However, its advantage to easily allow the genetic refreshment is of great importance as it can ensure the mass production of high-quality males and enhanced efficacy of operational SIT programs.
    Trvalý link: https://hdl.handle.net/11104/0349870

     
     
Počet záznamů: 1  

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