Počet záznamů: 1
Recent developments in capillary and microchip electroseparations of peptides (2021-mid-2023)
- 1.0575515 - ÚOCHB 2025 RIV US eng J - Článek v odborném periodiku
Kašička, Václav
Recent developments in capillary and microchip electroseparations of peptides (2021-mid-2023).
Electrophoresis. Roč. 45, 1/2 (2024), s. 165-198. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA ČR(CZ) GA20-03899S
Institucionální podpora: RVO:61388963
Klíčová slova: capillary electrochromatography * capillary electrophoresis * peptides * proteins * review
Obor OECD: Analytical chemistry
Impakt faktor: 3, rok: 2023
Způsob publikování: Open access
https://doi.org/10.1002/elps.202300152
This review article brings a comprehensive survey of developments and applications of high-performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.
Trvalý link: https://hdl.handle.net/11104/0345279
Počet záznamů: 1