Synthesis of layered dendrimers for bioapplications and material chemistry

0575379 - ÚCHP 2024 CZ eng D - Dizertace
Müllerová, Monika
Synthesis of layered dendrimers for bioapplications and material chemistry.
Ústav chemických procesů AV ČR, v. v. i. Obhájeno: Praha. 30.06.2022. - Praha, 2022. 133 s.
Institucionální podpora: RVO:67985858
Klíčová slova: dendrimer * drug delivery * glycoconjugate
Obor OECD: Organic chemistry

Advances in biomedical research require the development of biocompatible and specific synthetic glycoconjugates. This dissertation covers (i) robust methods for the synthesis of carbosilane dendritic structures for multivalent presentation of carbohydrate units, and ii) biophysical and biological evaluation of the compounds for their use in biomedical appli-cations. Six series of carbosilane glycodendrimers with glucose and galactose moieties were prepared and thoroughly characterized by analytical and physical-chemical methods common in the field. In vitro cytotoxicity assays revealed exceptional biocompatibility of the glycodendrimers irrespective of their generation and series. The application potential of the prepared compounds in anticancer therapy was investigated by encapsulating the therapeutic drug doxorubicin. Testing of the resulting dendrimer-drug complexes on MCF-7 and A2780 cancer cell lines showed their promising generation- and concentration-dependent anticancer activity, as well as pH-dependent drug release.
Trvalý link: https://hdl.handle.net/11104/0345182