Variability of fluorescence intensity distribution measured by flow cytometry is influenced by cell size and cell cycle progression

0574519 - BFÚ 2024 RIV US eng J - Článek v odborném periodiku
Fedr, Radek - Kahounová, Zuzana - Remsik, J. - Reiterova, M. - Kalina, T. - Souček, Karel
Variability of fluorescence intensity distribution measured by flow cytometry is influenced by cell size and cell cycle progression.
Scientific Reports. Roč. 13, č. 1 (2023), č. článku 4889. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GA20-22984S; GA ČR(CZ) GA21-11585S; GA MZd(CZ) NV18-08-00245
Institucionální podpora: RVO:68081707
Klíčová slova: AUTOFLUORESCENCE * METABOLISM * DNA
Obor OECD: Immunology
Impakt faktor: 4.6, rok: 2022
Způsob publikování: Open access
https://www.nature.com/articles/s41598-023-31990-1

The distribution of fluorescence signals measured with flow cytometry can be influenced by several factors, including qualitative and quantitative properties of the used fluorochromes, optical properties of the detection system, as well as the variability within the analyzed cell population itself. Most of the single cell samples prepared from in vitrocultures or clinical specimens contain a variable cell cycle component. Cell cycle, together with changes in the cell size, are two of the factors that alter the functional properties of analyzed cells and thus affect the interpretation of obtained results. Here, we describe the association between cell cycle status and cell size, and the variability in the distribution of fluorescence intensity as determined with flow cytometry, at population scale. We show that variability in the distribution of background and specific fluorescence signals is related to the cell cycle state of the selected population, with the 10% low fluorescence signal fraction enriched mainly in cells in their G0/G1 cell cycle phase, and the 10% high fraction containing cells mostly in the G2/M phase. Therefore we advise using caution and additional experimental validation when comparing populations defined by fractions at both ends of fluorescence signal distribution to avoid biases caused by the effect of cell cycle and cell size.
Trvalý link: https://hdl.handle.net/11104/0350079