The influence of amphiphilic carbosilane dendrons on lipid model membranes

0573511 - ÚCHP 2024 RIV NL eng J - Článek v odborném periodiku
Wróbel, D. - Edr, Antonín - Zemanová, E. - Strašák, Tomáš - Semerádtová, A. - Malý, J.
The influence of amphiphilic carbosilane dendrons on lipid model membranes.
Chemistry and Physics of Lipids. Roč. 255, SEP 2023 (2023), č. článku 105314. ISSN 0009-3084. E-ISSN 1873-2941
Grant CEP: GA ČR(CZ) GA20-21421S; GA MŠMT(CZ) LTC19049
GRANT EU: European Commission(XE) CA17140 - COST Action
Institucionální podpora: RVO:67985858
Klíčová slova: amphiphilic dendrons * liposomes * model membranes * self-assembling
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.4, rok: 2022
Způsob publikování: Open access s časovým embargem

The aim of the presented work was to study the interaction of two types of amphiphilic carbosilane dendritic structures (denoted as DDN-1 and DDN-2) suitable for further modification with streptavidin (DDN-1) or using click-chemistry approach (DDN-2), with selected neutral and negatively charged lipid model membranes, partially mimicking the basic properties of natural EXs biomembranes. To meet the goal, a number of biophysical methods were used for determination of the degree and mechanisms of the interaction. The results showed that the strength of interactions of amphiphilic dendrons with liposomes was related with surface charge of liposomes. Several steps of interactions were disclosed. The initialization step was mainly coupled with amphiphilic dendrons - liposomes surface interaction resulting in destabilization of large self-assembled amphiphilic dendrons structures. Such destabilization was more significant with liposomes of higher negative charge. With increasing concentration of amphiphilic dendrons in a solution the interactions were taking place also in the hydrophobic part of bilayer. Further increase of nanoparticle concentration resulted in a gradual dendritic cluster formation in a lipid bilayer structure.
Trvalý link: https://hdl.handle.net/11104/0343944