The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide

0573230 - ÚŽFG 2024 RIV NL eng J - Článek v odborném periodiku
Tejkalová, H. - Jakob, L. - Kvasnová, Simona - Klaschka, Jan - Sechovcová, Hana - Mrázek, Jakub - Páleníček, T. - Olša Fliegerová, Kateřina
The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide.
Heliyon. Roč. 9, č. 4 (2023), č. článku e15417. ISSN 2405-8440. E-ISSN 2405-8440
Grant CEP: GA MZd(CZ) NV17-31852A
Institucionální podpora: RVO:67985904 ; RVO:67985807
Klíčová slova: rats * lipopolysaccharide * antibiotics * behavior * microbiome
Obor OECD: Microbiology; Neurosciences (including psychophysiology (UIVT-O)
Impakt faktor: 4, rok: 2022
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S2405844023026245?via%3Dihub#sec5

The present study investigated whether neonatal exposure to the proinflammatory endotoxin lipopolysaccharide (LPS) followed by an antibiotic (ATB)-induced dysbiosis in early adulthood could induce neurodevelopmental disorders-like behavioral changes in adult male rats. Combining these two stressors resulted in decreased weight gain, but no significant behavioral abnormalities were observed. LPS treatment resulted in adult rats' hypoactivity and induced anxiety-like behavior in the social recognition paradigm, but these behavioral changes were not exacerbated by ATB-induced gut dysbiosis. ATB treatment seriously disrupted the gut bacterial community, but dysbiosis did not affect locomotor activity, social recognition, and acoustic reactivity in adult rats. Fecal bacterial community analyses showed no differences between the LPS challenge exposed/unexposed rats, while the effect of ATB administration was decisive regardless of prior LPS exposure. ATB treatment resulted in significantly decreased bacterial di-versity, suppression of Clostridiales and Bacteroidales, and increases in Lactobacillales, Enter-obacteriales, and Burkholderiales. The persistent effect of LPS on some aspects of behavior suggests a long-term effect of early toxin exposure that was not observed in ATB-treated animals. However, an anti-inflammatory protective effect of ATB cannot be assumed because of the increased abundance of pro-inflammatory, potentially pathogenic bacteria (Proteus, Suttrella) and the elimination of the bacterial families Ruminococcaceae and Lachnospiraceae, which are gener-ally considered beneficial for gut health.
Trvalý link: https://hdl.handle.net/11104/0343699