An application of UPLC-QQQ MS to characterize changes in free cholesterol and cholesterol esters in lung cells and lung tissue after mice exposure to metal oxide nanoparticles

0573127 - ÚIACH 2024 CZ cze A - Abstrakt
Laštovičková, Markéta - Kristeková, Daniela - Křůmal, Kamil - Coufalík, Pavel - Alexa, Lukáš - Buchtová, Marcela - Mikuška, Pavel
An application of UPLC-QQQ MS to characterize changes in free cholesterol and cholesterol esters in lung cells and lung tissue after mice exposure to metal oxide nanoparticles.
Eleventh Annual Conference of The Czech Society for Mass Spectrometry. Book of Abstracts. Olomouc: Česká společnost pro hmotnostní spektrometrii, 2023. s. 46-46. ISBN 978-80-907478-2-1.
[Annual Conference of The Czech Society for Mass Spectrometry /11./. 19.06.2023-21.06.2023, Brno]
Institucionální podpora: RVO:68081715 ; RVO:67985904
Klíčová slova: nanoparticles * LC-MS * cholesterols
Obor OECD: Analytical chemistry

The inhalation of lead oxide nanoparticles (PbO NPs) severely damaged target organs of exposed mice. Our optimized LC-MS method was used to compare the profile of lipids extracted from a control lung cell line (and lung tissue from control mice) compared to lung cells and tissue from mice exposed to elevated concentrations of airborne PbO NPs. Our study revealed a possible association of alteration of cholesterol metabolism after exposure to metal NPs.
Trvalý link: https://hdl.handle.net/11104/0343596