Počet záznamů: 1
Hormonal homeostasis associated with effective induction of triticale microspore embryogenesis
- 1.0572850 - ÚEB 2024 RIV DE eng J - Článek v odborném periodiku
Juzoń-Sikora, K. - Nowicka, Anna - Plačková, Lenka - Doležal, Karel - Żur, I.
Hormonal homeostasis associated with effective induction of triticale microspore embryogenesis.
Plant Cell, Tissue and Organ Culture. Roč. 152, č. 3 (2023), s. 583-604. ISSN 0167-6857. E-ISSN 1573-5044
Grant CEP: GA MŠMT(CZ) EF16_019/0000738
Institucionální podpora: RVO:61389030
Klíčová slova: Auxins * Cytokinins * Microspore reprogramming * Ovary co-culture
Obor OECD: Biochemical research methods
Impakt faktor: 2.3, rok: 2023
Způsob publikování: Open access
https://doi.org/10.1007/s11240-022-02433-y
The endogenous level of cytokinins (CKs) and auxins (Auxs) was analyzed in isolated microspores and ovaries of two doubled haploid (DH) lines of triticale (× Triticosecale Wittm.) to better understand the mechanism of microspore embryogenesis (ME)—the most efficient and widely applied method of producing DHs. The responsiveness of the studied lines to ME significantly varied. ME was induced by pre-treating tillers with low temperature (4 °C for 3 weeks) alone or in combination with synthetic auxin (2,4-D), auxin inhibitor (PCIB) or melatonin (MEL) applied for 4 days before microspore isolation. Hormonal profile analyses, accompanied by ME effectiveness evaluation confirmed the multi-level crosstalk of Auxs/CKs and the specific hormonal homeostasis required for effective microspore reprogramming. It was found that triticale microspores contained mainly cis zeatin derivatives: cis-zeatin-O-glucoside (cZOG), cis-zeatin riboside (cZR) and cis-zeatin (cZ), as well as indole-3-acetic acid (IAA) and IAA-aspartate (IAAsp). Increased ME efficiency was associated with higher contents of most of the identified CKs and Auxs, as well as the higher active Aux/active CK ratio. Trans CK isoforms were detected only in ovaries, confirming their importance as a source of bioactive molecules stimulating embryogenic development. Two of the pre-treatments tested: 12.5 μM PCIB and 50 μM MEL decreased the active Aux/active CK ratio, which was accompanied by an increase in the regeneration efficiency.
Trvalý link: https://hdl.handle.net/11104/0343408
Název souboru Staženo Velikost Komentář Verze Přístup 2023_Juzon-Sikora_PLANT CELL TISSUE AND ORGAN CULTURE_583.pdf 2 2.3 MB Jiná povolen
Počet záznamů: 1