The role of repetitive sequences in repatterning of major ribosomal DNA clusters in Lepidoptera

0572837 - BC 2024 RIV GB eng J - Článek v odborném periodiku
Dalíková, Martina - Provazníková, Irena - Provazník, J. - Groft-Tisza, P. - Pepi, A. - Nguyen, Petr
The role of repetitive sequences in repatterning of major ribosomal DNA clusters in Lepidoptera.
Genome Biology and Evolution. Roč. 15, č. 6 (2023), č. článku evad090. ISSN 1759-6653. E-ISSN 1759-6653
Grant CEP: GA MŠMT(CZ) LM2015047
Grant ostatní: GA ČR(CZ) GJ20-20650Y; GA MŠk(CZ) LM2015042; GA MŠk(CZ) LM2015085
Institucionální podpora: RVO:60077344
Klíčová slova: satellite * major ribosomal RNA genes * mobile elements
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 3.3, rok: 2022
Způsob publikování: Open access
https://academic.oup.com/gbe/article-pdf/15/6/evad090/50569543/evad090.pdf

Genes for major ribosomal RNAs (rDNA) are present in multiple copies mainly organized in tandem arrays. The number and position of rDNA loci can change dynamically and their repatterning is presumably driven by other repetitive sequences. We explored a peculiar rDNA organization in several representatives of Lepidoptera with either extremely large or numerous rDNA clusters. We combined molecular cytogenetics with analyses of second- and third-generation sequencing data to show that rDNA spreads as a transcription unit and reveal association between rDNA and various repeats. Furthermore, we performed comparative long read analyses among the species with derived rDNA distribution and moths with a single rDNA locus, which is considered ancestral. Our results suggest that satellite arrays, rather than mobile elements, facilitate homology-mediated spread of rDNA via either integration of extrachromosomal rDNA circles or ectopic recombination. The latter arguably better explains preferential spread of rDNA into terminal regions of lepidopteran chromosomes as efficiency of ectopic recombination depends on the proximity of homologous sequences to telomeres.
Trvalý link: https://hdl.handle.net/11104/0349805