An Evolutionary and Environmental Perspective of the Interaction of Nanomaterials with the Immune System-The Outcomes of the EU Project PANDORA

0572819 - MBÚ 2024 RIV CH eng M - Část monografie knihy
Navarro Pacheco, Natividad Isabel - Roubalová, Radka - Semerád, Jaroslav - Grasserová, Alena - Benada, Oldřich - Kofroňová, Olga - Cajthaml, Tomáš - Dvořák, Jiří - Bilej, Martin - Procházková, Petra
In vitro interactions of TiO2 Nanoparticles with earthworm coelomocytes: immunotoxicity assessment. Nanomaterials.
An Evolutionary and Environmental Perspective of the Interaction of Nanomaterials with the Immune System-The Outcomes of the EU Project PANDORA. Basel: MDPI, 2022 - (Boraschi, D.), s. 49-65. ISBN 978-3-0365-3948-5
Grant CEP: GA MZe(CZ) QK1910095
GRANT EU: European Commission(XE) 671881 - PANDORA
Institucionální podpora: RVO:61388971
Klíčová slova: earthworm * coelomocyte * reactive oxygen species * innate immunity * lipid peroxidation * alkaline comet assay * phagocytosis * apoptosis * gene expression
Obor OECD: Immunology
https://www.mdpi.com/2079-4991/11/1/250

Titanium dioxide nanoparticles (TiO2 NPs) are manufactured worldwide. Once they arrivein the soil environment, they can endanger living organisms. Hence, monitoring and assessing theeffects of these nanoparticles is required. We focus on the Eisenia andrei earthworm immune cellsexposed to sublethal concentrations of TiO2 NPs (1, 10, and 100 μg/mL) for 2, 6, and 24 h. TiO2 NPs
at all concentrations did not affect cell viability. Further, TiO2 NPs did not cause changes in reactiveoxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity.Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO2 NPsat the cellular level. At the gene expression level, slight changes were detected. Metallothionein,fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to10 μg/mL TiO2 NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells.TiO2 NPs were detected on coelomocyte membranes. However, our results do not show any strongeffects of these nanoparticles on coelomocytes at both the cellular and molecular levels.

Trvalý link: https://hdl.handle.net/11104/0343377