Quantification of interaction frequency between antigenpresenting cells and T cells by conjugation assay

0572809 - MBÚ 2024 RIV CZ eng J - Článek v odborném periodiku
Černý, Ondřej
Quantification of interaction frequency between antigenpresenting cells and T cells by conjugation assay.
Methods in Cell Biology. Roč. 173, č. 2023 (2023), s. 65-75. ISSN 0091-679X
Grant CEP: GA ČR(CZ) GA22-05356S
Institucionální podpora: RVO:61388971
Klíčová slova: Immunological synapse * Conjugation assay * Flow cytometry * Confocal microscopy * MutuDC * B3Z
Obor OECD: Microbiology
Impakt faktor: 1.829, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/abs/pii/S0091679X22001066?via%3Dihub

Interaction between an antigen-presenting cell and a T cell, and their subsequent conjugation are a prerequisite for the formation of the immunological synapse and productive, antigen-dependent activation of T cells. This initial interaction is accompanied by recognition of the presented antigen by the T cell receptor, and by changes in the morphology of the interacting cells and in actin cytoskeleton structure in the site of interaction. The experimental protocol below describes a simple assay for quantitative assessment of antigen-presenting cells-T cell conjugation using confocal microscopy or flow cytometry.
Trvalý link: https://hdl.handle.net/11104/0343369